Premium
The Polyclonal B Cell Activator Dextran‐Sulphate Induces Formation of Colony Stimulating Activity
Author(s) -
GRANSTRÖM M.,
WAHREN B.,
GRONOWICZ E.,
MÖLLER G.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00455.x
Subject(s) - myeloid , spleen , bone marrow , biology , colony stimulating factor , activator (genetics) , microbiology and biotechnology , stem cell , immunology , haematopoiesis , biochemistry , receptor
The effect of dextran‐sulphate (DS), a polyclonal activator known to stimulate immature murine B‐cells, was assayed in a culture system allowing the growth of myeloid cells. It was known that DS induced the production of a myeloid colony stimulating factor (CSF) by cells from both spleen and bone marrow. Nylonwool purified mouse spleen cells, enriched for T cells, showed a dimished CSF production in response to DS, while CSF production in response to Con A was increased. Furthermore, DS induced CSF in both spleen and bone marrow cells from nude mice. Removal of macrophages did not affect CSF production. The CSF induced was non‐dialysable and no small molecular weight or lipoprotein inhibitors could be demonstrated. The results suggest that DS activates cells other than T cells or macrophages (possibly B cells or null cells) to produce a myeloid stem cell stimulating substance. These results indicate that interactions between lymphoid and myeloid cells can take place during differentiation.