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Reactions and Crossreactions of a Rabbit Anti‐H2 Antigen Serum
Author(s) -
KVIST S.,
ÖSTBERG L.,
PETERSON P. A.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00454.x
Subject(s) - antiserum , antigen , antibody , microbiology and biotechnology , biology , epitope , glycoprotein , papain , rabbit (cipher) , chemistry , biochemistry , immunology , enzyme , statistics , mathematics
A rabbit antiserum raised against highly purified, papain‐solubilized H‐2 d antigens contained two sets of non‐crossreacting antibodies directed against each one of the two H‐2 antigen sub‐units. The antiserum recognized only 12,000 and 47,000 dalton polypeptide chains when spleno‐cyte membrane glycoproteins were analysed. Among the molecules precipitated with the rabbit antiserum all H‐2K and D antigens were present. In addition to regular H‐2K and D antigens minor amounts of material with the typical H‐2 antigen subunit structure, but lacking alloantigenic determinants, were precipitated by the antiserum. These ‘non‐H‐2 antigens’ were produced in relatively greater amounts by T‐cells than by B‐cells. Both sets of antibodies in the rabbit antiserum reacted with the TL antigens demonstrating that there is an immunological crossreactivity between the classical alloantigenic H‐2 antigen chain and the alloantigenic TL antigen chain. The F9 cell line, believed to represent cells at the morula stage, display H‐2 antigen‐like structures as revealed by the rabbit antiserum.