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D‐Penicillamine and Macrophages: Modulation of Lymphocyte Transformation by Concanavalin A
Author(s) -
BINDERUP L.,
BRAMM E.,
ARRIGONIMARTELLI E.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00453.x
Subject(s) - penicillamine , concanavalin a , macrophage , thymidine , lymphocyte , chemistry , endocrinology , medicine , microbiology and biotechnology , biology , immunology , biochemistry , in vitro
Pretreatment with d ‐Penicillamine of rat peritoneal macrophages markedly influences their ability to modulate Concanavalin A stimulated [ 3 H]thymidine incorporation in non‐adherent rat lymph node cells. Untreated macrophages added in high numbers to the lymphocytes (ratio lymphocytes/macrophage 7.5:1) depress their [ 3 H]thymidine incorporation. Pretreatment with d ‐Penicillamine further reduces this response. Lower proportions of untreated macrophages (ratio 15:1) exert a stimulatory effect on the lymphocytes. This response is reversed by pretreatment of macrophages with d ‐Penicillamine. Addition of untreated macrophages in very low proportions (40:1 and 75:1) is without effect on the [ 3 H]thymidine incorporation by the lymphocytes. This response is markedly stimulated by pretreatment of the macrophages with d ‐Penicillamine. The culture of macrophages in the presence of D‐Penicillamine increases their incorporation of [ 3 H] d ‐glucosamine, but it is without effect on their incorporation of [ 3 H] l ‐leucine. It is suggested that d ‐Penicillamine enhances the functional activity of the macrophage in the regulation of the lymphocyte response to mitogens.