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Rat Memory T Lymphocytes: in Vitro Proliferation Induced by Antigens of Actinomyces viscosus
Author(s) -
BURCKHARDT J. J.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00440.x
Subject(s) - phytohaemagglutinin , in vitro , antigen , in vivo , stimulation , biology , microbiology and biotechnology , extracellular , t cell , immunology , chemistry , immune system , biochemistry , endocrinology
Peripheral T lymphocytes from RIC‐Sprague‐Dawley rats primed in vivo several months earlier with Actinomyces viscosus Ny 1 reacted with a strong proliferative response upon antigenic challenge in vitro. Two different antigen preparations from A. viscosus Ny 1, a broken cell supernatant (BCS) and an extracellular fraction (EXC) gave maximum responses as measured by the uptake of [ 3 H] thymidine at a concentration of 10 μg at day 4 of culture. Phytohaemagglutinin (PHA) used as a control provoked a maximum proliferation in the same order of magnitude. T lymphocytes from unprimed. germfree and conventional animals showed a similar stimulation to PHA, but only a marginal reaction to BCS, and were not at all activated by EXC. Evidence is thus presented for efficient removal of cells responding non‐specifically to B‐cell mitogens.