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Irreversible Immunological Tolerance to Thymus‐independent Antigens is Restricted to the Clone of B Cells having both Ig and PBA Receptors for the Tolerogen
Author(s) -
FERNANDEZ C.,
MÖLLER G.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00436.x
Subject(s) - clone (java method) , receptor , epitope , antigen , antibody , spleen , dextran , dextranase , microbiology and biotechnology , immunology , clonal deletion , immune tolerance , biology , chemistry , t cell , immune system , t cell receptor , biochemistry , dna
Mice were tolerized to the α1–6 epitope of native dextran. When their spleen cells wore removed and activated by LPS, they did not synthesize anybodies against the tolerogen. However, when cells from tolerant mice were treated with dextranase or left untreated in culture for 24 h they were activated by LPS to the synthesis of antibodies against the tolerogen When 24 h tolerized lymphocytes were treated with dextranase and transferred with immunogenic doses of dextran to irradiated mice they failed to produce antibodies against the tolerogen. In contrast, cells incubated with dextran for 2 h and thereafter dextranase treated were readily immunized by dextran in the same system It is concluded that only the B cell clones having both I preceptors and PBA receptors for the tolerogen become irreversibly tolerized, whereas B cells having Ig receptors for a different PBA are not tolerized, but remain in a resting state, even though their Ig receptors have bound the tolerogen.