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Certain Rheumatoid Factors React with both IgG and an Antigen Associated with Cell Nuclei
Author(s) -
HANNESTAD K.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00435.x
Subject(s) - titer , antibody , rheumatoid factor , antigen , microbiology and biotechnology , agarose , chemistry , conjugate , immunology , medicine , biology , mathematical analysis , mathematics
Rheumatoid factors (RF) were isolated by their affinity for human IgG. RF from three out or twenty‐four sera (12%) with a RF‐latex titre 20 and an anti‐nuclear antibody (ANA) titre 250 cross‐reacted with nuclei from mouse cells. This antibody which represents an unknown proportion of RF, is designated RF/ANA. Only one of the three sera (Nil) contained large amounts of RF/ANA. A fourth scrum (Jos) had 6.6 mg of RF per ml and an ANA‐titre of 4096; all the ANA‐activity of this serum was absorbed by an IgG–agarose conjugate. Pan of the RF‐activity of serum Jos was carried by antigenically deficient IgM. The ANA‐activity of purified RF/ANA from four sera was inhibited by low concentrations of aggregated human IgG and by higher concentrations of human Fcγ fragments and non‐aggreated mouse anti‐DNP anybodies. The data provide strong support for the conclusion that certain antibody molecules can combine with both IgG and an antigen in cell nuclei.