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Functional Heterogeneity of Splenic T Lymphocyte Subpopulations I. Determination of Splenic Subpopulations by the Use of Mitogenic Probes
Author(s) -
WATERFIELD J. D.,
EKSTEDT R. D,
MÖLLER G.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb02141.x
Subject(s) - concanavalin a , in vivo , spleen , in vitro , lectin , microbiology and biotechnology , biology , immunology , splenocyte , receptor , chemistry , biochemistry
The results reported here suggest that there exist two T‐cell‐mitogenic responsive populations in the spleen—one that is responsive to concanavalin A (Con A) and a. second one that is responsive to Con A and phytohemagglutinin (PHA). Using an in vitro restimulation system, we have found that a primary in vivo activation with Con A effectively suppresses the subsequent restimulation response to PHA. However, in the reverse experiment, a primary in vivo PHA activation had little effect on the ability of tells to be restimulated with Con A. The suppression seen was due to a refractory recovery period displayed by all cells after previous activation and not to a nonspecific steric hindrance of mitogenic activation receptors due to the lectin remaining on the cell surfaces.