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In Vitro TNP‐Specific Antibody Formation by Peripheral Lymphocytes from Patients with Systemic Lupus Erythematosus
Author(s) -
MORIMOTO C.,
ABE T.,
HARA M.,
HOMMA M.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb02135.x
Subject(s) - antibody , immunology , in vitro , peripheral , lupus erythematosus , medicine , chemistry , biochemistry
Immunological reactivity in patients with systemic lupus erythematosus (SLE) was assessed by investigating in vitro trinitrophenyl (TNP)‐specific antibody formation by peripheral lymphocytes. Peripheral lymphocytes from 16 patients with SLE were cultured with TNP conjugated with horse erythrocytes (TNP–HRBC) in the presence of 2‐mercaptoethanol. The hemolytic plaque assay was used to detect hapten (TNP)‐specific antibody‐forming cells. Peripheral lymphocytes from normal individuals failed to produce antibody to TNP, whereas SLE lymphocytes produced a significant number of plaque‐forming cells. Co‐culture experiments with SLE and normal lymphocytes suggested that patients with SLE have a defect in T lymphocytes, leading to abnormal antibody production.