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Apparent Lack of Gene Dosage Effect in the PLT Assay
Author(s) -
JARAMILLO S.,
ANHORN G.,
SCHUNTER F.,
WERNET P.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb02116.x
Subject(s) - human leukocyte antigen , incubation , lymphocyte , gene dosage , typing , immunology , gene , biology , microbiology and biotechnology , chemistry , gene expression , genetics , antigen , biochemistry
In this paper the results of primed lymphocyte typing (PLT) tests are reported which show the response to HLA‐D‐specific stimulator and to unrelated cells. The aim was to find out the sensitivity of the PLT method for evaluating not only qualitative HLA‐D‐region differences but also gene dosage effects. In addition, the primed cells were restimulated with cells from HLA‐D‐homozygous individuals mutually negative with the specific stimulator in the usual 5‐day primary mixed lymphocyte culture incubation. No gene dosage effect in PLT experiments could be observed.