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Selection of Haploid Spermatozoa and Its Application to HLA‐D Typing
Author(s) -
FESTENSTEIN H.,
HALIM K.,
ARNAIZVILLENA A.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb02113.x
Subject(s) - biology , human leukocyte antigen , ploidy , typing , immunology , sperm , antiserum , transplantation , complement dependent cytotoxicity , genetics , antigen , cytotoxicity , in vitro , gene , medicine , antibody dependent cell mediated cytotoxicity
The expression of HLA‐A, ‐B, and ‐D determinants on the surface of spermatozoa is haploid. Using cytotoxicity and appropriate HLA antisera and complement, we were able to select haploid spermatozoa. The surviving sperms behaved similarly to homozygous lymphoid cells when cultured in mixed sperm lymphocyte culture (SLC). This method is being applied to define new HLA‐D specificities, to improve typing and matching for bone marrow transplantation and kidney transplantation and to study their association with various diseases.