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Specific Suppression of MLC and CML by Anti‐Idiotypic Antibodies in the Mouse
Author(s) -
WIGHT E.,
BINZ H.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00417.x
Subject(s) - antibody , lymphoblast , population , antiserum , antigen , concanavalin a , microbiology and biotechnology , epitope , immunology , c57bl/6 , incubation , biology , lymphocyte , andrology , cell culture , medicine , in vitro , genetics , biochemistry , environmental health
Rabbit antisera directed against idiotypic determinants of alloreactive mouse CBA anti‐C57BL/6 T blasts were raised in the following manner: first, a rabbit serum directed against nonspecific CBA blasts cells was prepared by injecting CBA concanavalin A blasts three times at monthly intervals into a rabbit. Second, specific CBA anti‐C57BL/6 T lymphoblasts were induced in a mixed lymphocyte culture (MLC), were purified by gravity sedimentation through a fetal calf serum gradient, and. finally, were incubated with the anti‐blast serum from the first step. During this incubation, presumably all epitopes of the blast cell population were blocked by anti‐blast antibodies, except for the greatly amplified set of CBA anti‐C57BL/6 alloreactive idiotypes. The mixture was then Injected into fresh rabbits, which were boosted with similar mixtures after 3 and 6 weeks. Blood samples were removed 10 days after each injection. Such sera, when used together with complement, inhibited specifically the stimulation of CBA cells by C57BL/6 antigens in MLC and the CBA anti‐C57BL/6 killer cells.