Premium
Staphylococcal Protein A in Immunoferritin Techniques
Author(s) -
BÄCHI T.,
DORVAL G.,
WIGZELL H.,
BINZ H.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00390.x
Subject(s) - staphylococcus aureus , antibody , chemistry , ferritin , antiserum , antigen , protein a , biochemistry , microbiology and biotechnology , immunology , bacteria , biology , genetics
Protein A (pA) of Staphylococcus aureus , by virtue of its reactivity with the Fc portion of a variety of mammalian immunoglobulins (IgG), can be used for electron‐microscopical immunoferritin technique. The conjugation of pA with ferritin (pA‐F) by means of bis (4 fluoro‐3‐nitrophenyl) sulfone results in a highly specific marker for IgG in indirect labeling experiments. Native pA can be used as a noncovalent bridging agent between Specific antibodies and antiferritin capturing the marker molecule. The use of pA and pA‐F is demonstrated with a model system involving human erythrocytes and twO rabbit antisera directed against different surface antigens of the human erythrocyte membrane.