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A T‐Cell Marker in Mouse Fibroblast × T‐Lymphocyte Somatic Cell Hybrids
Author(s) -
SARAVIA N.,
DEMARS R.,
DUNLAP B.,
BACH F. H.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00374.x
Subject(s) - microbiology and biotechnology , gel electrophoresis , cell fusion , fibroblast , biology , cell culture , cell , polyacrylamide gel electrophoresis , somatic cell , somatic fusion , lymphocyte , sodium dodecyl sulfate , chemistry , biochemistry , gene , immunology , genetics , enzyme
Continuous hybrid cell lines have been generated by the fusion of allogeneically primed marine T lymphocytes with mouse L‐cell‐derived fibroblasts of line 613. The resulting hybrid clones express one or more T‐cell‐specific surface components identifiable by radioiodination of intact cells followed by NP‐40 solubilization of membrane proteins and sodium dodecyl sulfate polyacrylamide gel electrophoresis. These markers are stably expressed by the hybrid lines after several months of passage.