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A Note on the Interpretation of Identity Reactions in Immunoelectrophoresis
Author(s) -
DANIELSEN M.,
SJÖSTRÖM H.,
NORÉN O.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00372.x
Subject(s) - immunoelectrophoresis , antigen , immunodiffusion , heterologous , identity (music) , neuraminidase , biology , electrophoresis , chemistry , immunology , biochemistry , philosophy , gene , virus , aesthetics
Immunoelectrophoretic methods, used in studies of the tissue specificity of two membrane‐bound peptidases obtained from pig, were observed to produce new types of reactions resembling those of partial immunochemical identity. In addition, the results obtained by fused rocket and rocket‐line immunoelectrophoresis suggested that the unshared antigenic determinants were situated on the heterologous antigens. When analyzed in the immunodiffusion system, the antigens showed a reaction of total immunochemical identity. Zone electrophoresis of the antigens demonstrated large differences in electrophoretic mobility. Neuraminidase treatment of the antigens almost abolished these differences, and the antigens now exhibited almost complete antigenic identity, suggesting that the precipitate patterns observed arose from the mobility differences.