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Suppression of Immunological Activities by Mouse Amniotic Fluid
Author(s) -
ETLINGER H. M.,
CHILLER J. M.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00363.x
Subject(s) - ficoll , polyclonal antibodies , microbiology and biotechnology , spleen , lipopolysaccharide , antigen , concanavalin a , immunology , antibody , biology , in vitro , chemistry , peripheral blood mononuclear cell , biochemistry
Mouse amniotic fluid (MAF) was shown to be capable of suppressing those antibody responses observed in euthymic or athymic mouse spleen cell cultures to the T‐independent antigens dinitrophenylated Ficoll (DNP‐Ficoll) and trini‐trophenylated lipopolysaccharide (TNP‐LPS) and to the polyclonal B‐cell activators LPS and purified protein derivative of tuberculin (PPD). Titration experiments demonstrated that the suppressive capacity of MAF for either LPS or DNP‐Ficoll responses was maintained up to a MAF dilution of 1:120. Pre‐incubation of spleen cells obtained from athymic mice with MAF for 8 h significantly suppressed polyclonal B‐cell activation of such cells induced by LPS, although suppression was greater when MAF was present during the entire culture period. In addition, the suppressive activity that MAF demonstrated for antibody production induced by DNP‐Ficoll or LPS was not lost as a result of dialysis. MAF also suppressed the secondary in vitro prolixferative responses of lymph node cells sensitized to the T‐dependent antigen human gamma globulin (HGG). HGG‐induced proliferation of such cells appeared to be more susceptible to suppression effected by MAF than concanavalin‐A‐induced proliferation.