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Studies on Interference with Tolerance Induction in T Cells
Author(s) -
FESSIA S. L.,
KONG Y. M.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00359.x
Subject(s) - spleen , population , bone marrow , suppressor , immunology , adjuvant , immune tolerance , biology , t cell , antigen , thymectomy , microbiology and biotechnology , immune system , medicine , biochemistry , myasthenia gravis , environmental health , gene
The mechanism of T‐cell tolerance to a thymus‐dependent antigen was examined, using the adjuvant polyadenylic‐polyuridylic acid (poly A:U). In adoptive transfer experiments, thymus cells obtained from donor mice 2 days after treatment with a tolerogenic dose of bovine γ‐globulin (sBGG) did not cooperate with bone marrow (BM) cells in irradiated recipients challenged with aggregated BGG (aBGG). In contrast, thymus cells from donors given sBGG plus poly A:U retained their helper activity, as assayed by hemagglutination and rosette formation of spleen cells. The effect of poly A:U in preventing tolerance induction was also demonstrable in the cortisone‐resistant population, in that thymus cells from cortisone‐treated donors that had received sBGG and poly A:U retained their helper function. The presence of suppressor cells and the effect of poly A:U on their stimulation were also examined. sBGG‐treated thymus cells suppressed the response of BGG‐primed spleen cells in lethally irradiated mice, whereas thymus cells from donors treated with sBGG and poly A:U were not suppressive. These observations show that poly A:U prevents tolerance induction and the development of suppressor activity in T cells.