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Anti‐Parental‐Strain Lymphocyte Responsiveness of F 1 Hybrid‐Strain Lymphocytes
Author(s) -
McCULLAGH P.
Publication year - 1977
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1977.tb00332.x
Subject(s) - lymphocyte , strain (injury) , spleen , biology , thoracic duct , lymph , immunology , lymphatic system , medicine , pathology , anatomy
The interactions between immunocompetent parental‐strain tells and the F 1 hybrid rat into which these cells have been injected were studied. This involved examination of the behavior of both parental‐ and host‐strain cells. Host lymphocytes reactive against parental‐strain lymphocytes appeared in the thoracic duct lymph within 12 h of the intravenous injection of parental thoracic duct lymphocytes or thymus cells. The activity of these F 1 hybrid‐strain lymphocytes was directed preferentially against parental‐strain lymphocytes with anti‐F 1 hybrid potential. This subpopulation of parental cells was absent from the thoracic duct but was well represented in the spleen, this enrichment being masked by a reversible F 1 hybrid anti‐parental‐strain lymphocyte response. If the anti‐parental‐strain lymphocyte activity of F 1 hybrid cells was interrupted by the destruction of host‐strain cells with antiserum, parental‐strain lymphocytes in the tissues of F 1 hybrid rats could be reactivated. By this procedure it was possible to passage graft‐versus‐host reactions initiated by parental‐strain lymphocytes through several F 1 hybrid rats, thereby implying that anti‐parental lymphocyte activity is of importance in limiting the serial passage of these reactions. Some of the anti‐parental lymphocyte activity observed on the part of F 1 hybrid‐strain cells was probably mediated by anti‐idiotypic antibodies, but other phenomena, especially selective migration of both donor and host lymphocyte subpopulations, are of major importance in the interaction between parental‐strain lymphocytes and an F 1 hybrid host.