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In Vitro Reactivity of Human Lymphocytes after Cryopreservation Using a Programmed Cooling Device
Author(s) -
BOIS M. J. G. J.,
SCHELLEKENS P. TH. A.,
WIT J. J. F. M.,
EIJSVOOGEL V. P.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03852.x
Subject(s) - cryopreservation , in vitro , reactivity (psychology) , microbiology and biotechnology , biology , chemistry , medicine , embryo , biochemistry , pathology , alternative medicine
. In this article a technique is described for the cryopreservation of human lymphocytes employing a programmed cooling device. A preset temperature curve was used which compensated for the heat exchange during crystallization. The influence of this method of lymphocyte preservation on a variety of the in vitro functions of these cells was evaluated by means such as the lymphocyte transformation test, CML and investigations for lymphocyte membrane markers. All the lymphocyte functions that were tested were found to be retained in full.