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Immunological Removal of Contaminants: a Useful Principle in Protein Purification Applied to Human Secretory Component and J Chain
Author(s) -
BRANDTZAEG P.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03838.x
Subject(s) - secretory component , component (thermodynamics) , colostrum , chain (unit) , j chain , chemistry , yield (engineering) , contamination , chromatography , heavy chain , biochemistry , biology , antibody , immunology , materials science , ecology , physics , astronomy , metallurgy , gene , thermodynamics
Summary. An outline is given of the purification of free secretory component (SC) from human colostrum and of J chain released from polymeric IgA by mild reduction. Immunological removal of impurities is a crucial purification step, but one that has to be combined with conventional biochemical methods. The yield of highly purified material was 10% for SC and 30% for J chain.