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Isolation and Characterization of Sea Star Factor
Author(s) -
PRENDERGAST R. A.,
LIU S. H.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03037.x
Subject(s) - isolation (microbiology) , star (game theory) , factor (programming language) , biology , computer science , microbiology and biotechnology , physics , astrophysics , programming language
Sea star factor (SSF) harvested from the echinoderm Asterias forbesi celomocytes is a potent inhibitor of the primary immune response to T‐dependent antigens and also suppresses concanavalin‐A‐induced mitogenesis. We have isolated and purified a basic protein from this source which has a molecular weight of approximately 38,000 daltons and is responsible for these biological activities. After complete reduction, SSF was shown to be composed of a single pair of heavy and light chains. Preincubation of spleen cells with concanavalin A does not inhibit binding of SSF; however, specific binding can be inhibited by preincubation with unlabeled SSF. These data suggest that the binding site for SSF is not the same as that for concanavalin A.