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Production of Monospecific Antisera Against Antigenic Components of Mycobacterium bovis (BCG)
Author(s) -
HARBOE M.,
CLOSS O.,
DEVERILL J.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03035.x
Subject(s) - antiserum , antigen , mycobacterium bovis , immunization , immunoelectrophoresis , microbiology and biotechnology , biology , mycobacterium , virology , epitope , immunology , mycobacterium tuberculosis , bacteria , medicine , tuberculosis , genetics , pathology
Monospecific antisera against four different antigenic components of Mycobacterium bovis (BCG) were produced by immunization of rabbits with precipitates cut out of gels after crossed immunoelectrophoresis (CIE). An important feature is to use a semipurified mycobacterial antigenic preparation, or a cross‐reactive antigen, to obtain a simpler pattern in CIE so that a clean precipitate, free from contaminating antigens, can be obtained for immunization. The findings with one particular antigen are shown, and the use of such antisera is discussed.