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PHA‐Induced Activation of Suppressor Cells in Normal Human Peripheral Blood Lymphocytes
Author(s) -
KURNICK J. T.,
BELL C.,
GREY H. M.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03026.x
Subject(s) - concanavalin a , pokeweed mitogen , mitogen activated protein kinase , stimulation , microbiology and biotechnology , biology , lymphocyte , peripheral blood , cell , immunology , chemistry , in vitro , biochemistry , endocrinology
Normal human peripheral blood and tonsil lymphocytes can be stimulated to proliferate by phytohemagglutinin (PHA). When cells cultured with this mitogen for 3 days were transferred fo fresh autologous lymphocytes in fresh medium with PHA, the mitogen response of the fresh lymphocytes was suppressed. The suppression required the presence of viable cells, in that culture supernatants alone were not inhibitory and cell extracts showed only marginal inhibition. Approximately equivalent numbers of previously stimulated cells were required to produce optimal suppression of the PHA response of fresh cells. Cells irradiated after PHA stimulation were as effective as nonirradiated cells in causing suppression. PHA‐stimulated cells also inhibited concanavalin A‐induced proliferation and a mixed lymphocyte reaction. However, PHA‐stimulated cells only partially inhibited the response to pokeweed mitogen. The suppressive effects were fully retained by a nylon‐wool‐enriched T‐cell fraction but not by a B‐cell‐enriched fraction.