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Studies with B‐Cell Allo‐ and Hetero‐Antisera: Parallel Reactivity and Special Properties
Author(s) -
WINCHESTER R. J.,
WANG C. Y.,
HALPER J.,
HOFFMAN T.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03024.x
Subject(s) - antiserum , antibody , antigen , population , biology , b cell , microbiology and biotechnology , cell , leukemia , receptor , immunology , medicine , biochemistry , environmental health
The expression of B‐cell antigens on various cell populations was studied through the use of human alloantisera and with heteroantisera raised to preparations of the alloantigen bearing molecules isolated from B‐cell lines. The allo‐ and heteroantisera competed with each other in blocking experiments and gave closely parallel results, reacting with normal and leukemic B lymphocytes, monocytes, K‐rosette‐negative acute lymphatic leukemias, all acute and certain chronic myclogenous leukemias, and a minor population of cells in fetal spleen and liver. These highly immunogenic surface components appeared to comprise the dominant B‐cell‐specific plasma membrane determinants. Neither type of antiserum reacted with any but a minor population of normal or pokeweed‐mitogen‐transformed T cells, fetal thymic lymphocytes, E‐rosette‐positive acute lymphatic leukemias, or Sezary‐cell leukemia. Through the use of these antisera evidence was obtained that Fc‐receptor‐bearing Ig‐negative lymphocytes were divisible into two groups according to the presence or absence of the B‐cell antigens. Both hetero‐ and allo‐antisera blocked binding of immune complexes or antibody‐coated ox erythrocytes to Fc receptors on B cells. F(ab') 2 , fragments of the hetero‐antibodies strongly inhibited antibody‐dependent cell‐mediated killing.