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IgG on Infants' B Lymphocytes: Enhanced Binding of IgG by IgM‐Bearing Lymphoid Cells in Early Childhood
Author(s) -
SIEGAL F. P.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03022.x
Subject(s) - immunoglobulin d , trypsinization , lymphocyte , antiserum , surface immunoglobulin , immunology , antibody , receptor , microbiology and biotechnology , biology , peripheral blood mononuclear cell , immunoglobulin m , immunoglobulin g , population , b 1 cell , b cell , chemistry , immune system , medicine , in vitro , t cell , trypsin , antigen presenting cell , biochemistry , enzyme , environmental health
IgM/IgD‐bearing lymphocytes (B cells) from children in the first few weeks of life were found to also have surface IgG, unlike most normal adult B cells. The IgG was loosely bound to the lymphocyte surface and was partially or completely removed by incubation at 37°C or by trypsinization. When F(ab) 2 antisera were employed, very few infant B cells had surface IgG, although the IgM staining was similar to that obtained with the native antisera. IgM/IgD‐positive cells bound IgG anti‐Rh‐coated (Ripley) erythrocytes, unlike most adult B lymphocytes. Capping experiments suggested that an Fc receptor on these cells could be redistributed by the anti‐IgM‐surface IgM complex. These data indicate that, during infancy, B‐lymphocyte receptors for IgG are of altered affinity, frequency, or availability compared with adult B‐lymphocyte Fc receptors and resemble the Fc receptors found on ‘third population’ (Fc + Ig ‐ ) mononuclear cells and monocytes.