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Bence Jones Proteins and Light Chains of Immunoglobulins XIV.
Author(s) -
SOLOMON A.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03018.x
Subject(s) - bence jones protein , antigenicity , immunoglobulin light chain , antiserum , chemistry , antigen , antibody , epitope , microbiology and biotechnology , biology , biochemistry , genetics
The region on the light chain molecule responsible for expression of the χ and λ antigenic determinants was determined by comparative immunochemical analyses of intact Bence Jones proteins and naturally occurring or enzymatically derived fragments of Bence Jones proteins that lacked extensive portions of the V region or part of the C region. The reactivity of these fragments with numerous antisera having specificity for light‐chain antigenic determinants indicated the essentiality of the intact light polypeptide chain for expression of the χ and λ antigenic determinants. The conformational dependency of the χ and λ antigenic determinants was also evidenced by denaturation‐renaturation studies on χ and λ chains. The V domain, C domain, and interdomain ‘switch’ region contribute to the expression of χ or λ antigenicity and to certain isotypic and allotypic specificities.