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Restriction of Human Immune Antibodies to Heavy‐Chain Variable Subgroups
Author(s) -
NATVIG J. B.,
FØRRE Ø.,
MICHAELSEN T. E.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb03016.x
Subject(s) - antibody , subclass , antigen , hemagglutination , immune system , biology , heavy chain , virology , microbiology and biotechnology , immunology
Human immune antibodies such as anti‐Rh and anti‐Kell antibodies were tested in hemagglutination and hemagglutination inhibition experiments for V H subgroup composition. A striking V H subgroup restriction was found in several of these groups of antibodies. In the majority of cases there was a restriction to one particular V H subgroup for a single antibody specificity in a given individual. In some cases there was also an overall restriction to one particular subgroup for antibodies with the same antibody specificity. This was particularly pronounced for anti‐D antibodies, which were primarily restricted to V H II, and for the anti‐Kell, which was particularly related to V H III. Subgroup‐specific antigens for all the main V H subgroups were blocked on combination of the antibody molecule with antigen. No relation was found between V H restriction and restriction to IgG subclass, or genetic markers or χ and λ light‐chain type.