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Detection of β 2 ‐Microglobulin in Sera from Diverse Vertebrate Species by the Use of a Heterologous Radioimmunoassay
Author(s) -
GORDON S. M.,
KINDT T. J.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb00305.x
Subject(s) - heterologous , radioimmunoassay , antiserum , biology , phylogenetic tree , vertebrate , cross reactions , microbiology and biotechnology , antibody , biochemistry , immunology , gene
The strong cross‐reaction between rabbit and human β 2 ‐microglobulins, as detected by goat antisera, has permitted the development of heterologous radioimmunoassays to detect homologues of β 2 ‐microglabulin (β 2 μ) in the sera of various vertebrate species. The assay based on the inhibition of the binding reaction between goat anti‐rabbit β 2 μ and human β 2 μ divided the sera examined into two groups, one giving high levels of inhibition (∼80%), and the other lower levels (∼20%). There was no evident correlation between this division and phylogenetic classification. When the same group of sera was tested using the assay based on the inhibition of binding of rabbit β 2 μ to goat anti‐human β 2 μ, a wide range of maximal levels of inhibition was observed, with clustering of phylogenetically related species