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Effects of Granulocyte Neutral Proteases on Complement Components
Author(s) -
JOHNSON U.,
OHLSSON K.,
OLSSON I.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb00296.x
Subject(s) - elastase , collagenase , immunoelectrophoresis , proteases , proteolysis , chemistry , biochemistry , neutral protease , protease , pancreatic elastase , complement component 5 , granulocyte , macroglobulin , microbial collagenase , peptide hydrolases , autolysis (biology) , trypsin , enzyme , microbiology and biotechnology , complement system , biology , immunology , antibody
The proteolytic effects of collagenase and elastase from human granulocytes were investigated on the human complement components C3 and C5 in serum and with purified components. The conversion of C3 was analyzed with crossed immunoelectrophoresis as described by Ganrot, and the conversion of C5 was detected with immunoelectrophoresis according to Scheidegger's method. Collagenase converted C3 to C3b but had no detectable effect on C5. Elastase converted C3 to C3b and converted C5 to a C5b‐like fragment. The proteolysis by collagenase and elastase in serum was not detectable until the molar ratio for enzyme to the protease inhibitors α 1 ‐antitrypsin and α 2 ‐macroglobulin was greater than 1.