Premium
Generation of T Memory Cells in One‐Way Mixed Lymphocyte Culture
Author(s) -
HÄYRY P.,
ANDERSSON L. C.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb00293.x
Subject(s) - ultraviolet light , mitomycin c , in vitro , spleen , antigen , lymphocyte , biology , microbiology and biotechnology , t lymphocyte , immunology , strain (injury) , chemistry , biochemistry , genetics , photochemistry , anatomy
Neither normal CBA (H‐2 k ) nor purified spleen T cells respond in vitro to soluble or insoluble membrane preparations or to ultraviolet‐light‐inactivated stimulator cells of the allogeneic DBAJ2 (H‐2 d ) strain. However, CBA spleen cells deprived of phagocytic cells show a slight proliferative response under these conditions. After being primed against mitomycin‐blocked DBAJ2 cells in one‐way mixed lymphocyte culture, the secondary blast‐derived T ‘memory’ cells display a good secondary blast (proliferative) response to both membrane antigens and to ultraviolet‐light‐inactivated stimulator cells. In addition to this, the secondary T lymphocytes—in contrast to nonprimed T cells—respond by cytotoxicity when ultraviolet‐light‐inactivated cells are used as the second stimulant.