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The Role of B‐Cell Memory in Secondary IgG and IgM Responses
Author(s) -
SEPPÄLÄ I.,
HURME M.,
SARVAS H.,
MÄKELÄ O.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb00272.x
Subject(s) - immunology , immunoglobulin m , antibody , immunoglobulin g , biology
Mice were primed with the hapten 3‐nitro‐4‐hydroxy‐5‐iodophenacetic acid (NIP) conjugated to chicken globulin (CG) and were boosted 2, 6, or 12 months later with CG conjugates of the related haptens 3,5‐diiodo‐4‐hydroxyphenacetic acid (DIP) or 3‐nitro‐4‐hydroxyphenacetic acid (NP). Accelerated secondary responses were demonstrated both in the 7S and 19S class. Fine‐specificities of secondary‐response antibodies were studied by the hapten inhibition method of haptenated bacteriophage inactivation. 7S antibodies were found to have the fine‐specificity of anti‐NIP antibodies regardless of whether DIP or NP was the booster hapten (‘original antigenic sin’). 19S antibodies had the fine‐specificity of anti‐DIP when DIP was the booster hapten. NP as the booster hapten retailed in 195 antibodies whose fine‐specificity was intermediate between anti‐NIP and anti‐NP. A strong B‐cell memory could thus be demonstrated in the 7S antibody response and a weak B‐cell memory in the 19S antibody response.