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Distribution of Heavy‐Chain Variable‐Region (V H ) Subgroups on Human Lymphocytes
Author(s) -
FØRRE Ø.,
NATVIG J. B.,
FRØLAND S. S.,
JOHNSON P. M.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb00266.x
Subject(s) - antiserum , staining , microbiology and biotechnology , fluorescein isothiocyanate , population , immunofluorescence , lymphocyte , indirect immunofluorescence , biology , chemistry , antibody , immunology , pathology , medicine , fluorescence , physics , environmental health , quantum mechanics
Lymphocytes from 20 normal blood donors were stained with fluorescein isothiocyanate (FITC)‐conjugated anti‐F(ab′) 2 , anti‐V H I, anti‐V H II, and anti‐V H III subgroup antisera. The means of the percentages of staining were 2.9%, 5.0%, and 5.5% for the V H I, V H II, and V H III subgroups, respectively. The sum of the percentages of the lymphocytes stained with each of the V H subgroup‐specific antisera corresponded well with the percentages of lymphocytes stained with an anti‐F(ab′) 2 antiserum. In addition, it was shown by double immunofluorescence staining and in various depletion experiments and tests with thymocytes and lymphocytes from patients with hypogammaglobulinemia that the cells staining with anti‐V H antisera corresponded to the membrane Ig‐positive cells—that is, the B‐lymphocyte population.