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Arming of Lymphoid Cells by IgG Antibodies Treated with Protein A from Staphylococcus aureus
Author(s) -
SULICA A.,
LAKY M.,
GHERMAN M.,
GHETIE V.,
SJÖQUIST J.
Publication year - 1976
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1976.tb00262.x
Subject(s) - antibody , spleen , staphylococcus aureus , rosette (schizont appearance) , microbiology and biotechnology , fragment crystallizable region , protein a , immunoglobulin g , biology , chemistry , antigen , in vitro , immunology , bacteria , biochemistry , genetics
Mouse spleen lymphocytes treated with rabbit IgG anti‐sheep erythrocytes (SRBC) complexed with protein A of Stapbylococcus aureus (SpA) form rosettes with SRBC. The attachment of SRBC to lymphocytes was due to the binding of the SpA‐IgG antibody complex to the surface of the lymphocytes and was thus considered ‘arming’ of the cells. Normal mouse spleen cells ‘armed’ with SpA‐rabbit IgG anti‐chicken erythrocytes (CRBC) kill specifically 51 Cr‐labeled CRBC ‘in vitro’ in the absence of free antibodies. The killing by these ‘armed’ cells is an effect of the cell‐bound SpA‐IgG antibody complex. Both the SRBC rosette formation and the cell‐mediated CRBC killing was dependent on the concentration of the SpA‐IgG antibody complexes used for ‘arming’ the cell. A 100‐fold increase in rosette formation or in killing of target cells was recorded for lymphocytes treated with SpA‐IgG antibody complexes in comparison with cell treated with noncomplexed IgG antibodies. The specific binding of SpA‐IgG antibody complexes to the Fc receptors of mouse spleen cells was demonstrated by inhibition studies. More than 60% inhibition of the rosette formation and in the killing of target cells was shown for cell treated With normal rabbit IgG or its Fc fragment before addition of the SpA‐IgG antibody complex.