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Identification of Bovine Serum Proteins by Quantitative Immunoelectrophoretic Methods
Author(s) -
ANDERSEN METTE MARIE,
KRøLL JENS
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb03823.x
Subject(s) - immunoelectrophoresis , antiserum , blood proteins , electrophoresis , bovine serum albumin , chemistry , antibody , microbiology and biotechnology , biochemistry , biology , immunology
Line immunoelectrophoresis of bovine serum revealed a minimum of 37 bovine serum proteins. In line‐absorption immunoelectrophoresis, 13 of these proteins were identified by their cross‐reactivity with monospecific antisera against human serum proteins. One additional protein was identified by monospecific antisera against bovine immunoglobulins. Crossed immunoelectrophoresis revealed a minimum of 40 bovine proteins and demonstrated their electrophoretic mobility. In crossed‐line immunoelectrophoresis all 37 line precipitates were related to their corresponding peak precipitates and the electrophoretic mobility of the 14 proteins identificd was determined.