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Characterization of Bovine Whey Proteins by Crossed Immunoelectrophoresis
Author(s) -
LøWENSTEIN H.,
BJERRUM O. J.,
WEEKE EVA,
WEEKE B.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb03822.x
Subject(s) - bovine milk , immunoelectrophoresis , precipitin , whey protein , globules of fat , chemistry , antibody , chromatography , biochemistry , food science , biology , milk fat , immunology , linseed oil
A reference pattern obtained by crossed immunoelectrophoresis of bovine whey proteins from mature milk, using rabbit antibovine whey antibodies, has been worked out. Out of the total of 29 easily recognizable precipitates, 5 milk‐specific proteins and 21 serum proteins were identified. Furthermore, whey proteins which also occur in bovine milk fat globule membrane preparations and in extracts of bovine hair and dandruff were identified. The use of the obtained reference pattern for the identification of milk allergens and the determination of the specificity of human milk precipitins against bovine whey proteins is outlined.