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Detection of Biospecific Interaction during the First Dimension Electrophoresis in Crossed Immunoelectrophoresis *
Author(s) -
BøGHANSEN T. C.,
BJERRUM O. J.,
RAMLAU J.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb03820.x
Subject(s) - glycoprotein , immunoelectrophoresis , electrophoresis , lectin , gel electrophoresis , biochemistry , chemistry , microbiology and biotechnology , biology , genetics , antibody
Lectins were included in the first dimension gel in crossed immunoelectrophoresis of human serum proteins. Con A resulted in a retardation of most glycoproteins during the first electrophoresis and heterogeneous forms of individual glycoproteins could be detected. Ulex europeus lectin gave most of the proteins a higher migration velocity. Pokeweed mitogen did not interact with serum glycoproteins, but it did with serum lipoproteins. The method allows a close study of interacting components, determination of binding specificities, and detection of minor interacting components, including heterogeneous molecular forms.