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Continuous Growth of Mitogen‐Reactive B Lymphocytes
Author(s) -
MELCHERS F.,
COUTINHO A.,
HEINRICH G.,
ANDERSSON J.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb03728.x
Subject(s) - immunoglobulin d , secretion , microbiology and biotechnology , b cell , mitogen activated protein kinase , cell growth , immunology , chemistry , biology , antibody , endocrinology , biochemistry , signal transduction
B‐cell mitogens induce continous growth of murine lymphocytes in suspension cultures. Continuous growth is observed only at low cell densitiess, below 10 6 cells/ml, in regularly renewed medium containing β‐mercaptoethanol and a growth‐supporting fetal calf serum. Continuous growth of B lymphocytes in culture appears to be favored by conditions under which maturation of Ig‐secreting plaque‐forming cells does not occur. On the other hand, maturation is observed whenever stimulated B cells cease to divide. This maturation leads to IgM secretion followed by secretion of IgG‐ or IgD‐like molecules. Neither continuous growth of B cells nor the ‘switch’ to secretion of IgG‐ or IgD‐like molecules requires the presence of T cells.