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Detection of Measles Antibodies in Cerebrospinal Fluid and Serum by a Radioimmunoassay
Author(s) -
CUNNINGHAMRUNDLES C.,
JERSILD C.,
DUPONT B.,
POSNER J. B.,
GOOD R. A.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb03718.x
Subject(s) - antibody , cerebrospinal fluid , measles virus , measles , radioimmunoassay , complement fixation test , multiple sclerosis , antigen , immunology , serology , virology , medicine , pathology , vaccination , endocrinology
Evidence that different structural components of the measles virus may act as antigens has been provided by the serologic methods of hemagglutination inhi bition hemolysin inhibition, and nucleocapsid complement fixation. Using radio‐iodinated measles viral antigens, an immune precipitation assay has been designed that is capable of discriminating among various reactivities to measles viral structural components in serum or cerebrospinal fluid (CSF) and of distinguishing whether IgG and IgM antibody is involved. This technique has been applied to the study of measles antibodies in CSF and sera of patients with multiple sclerosis (MS) and other neurologic diseases. From data presented here, it was found that both groups of patients have individual reactivity to measles proteins, present in CSF and serum, whereas three normal CSF samples were found not to have such antibodies. It appears that oligoclonal immunoglobulins in CSF of MS patients may be detected by this method, and one patient with MS was found to have CSF IgM anti‐measles antibodies.