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Induction of Histamine Release and Desensitization in Human Leukocytes
Author(s) -
PETERSSON B.Å.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02686.x
Subject(s) - histamine , immunoglobulin e , desensitization (medicine) , chemistry , protein a , incubation , antibody , immunology , microbiology and biotechnology , biochemistry , biology , receptor , pharmacology
Histamine release from normal human leukocytes can be induced by anti‐IgE or protein A from Staphylococcus aureus. After incubation in buffer at 37°C for various time intervals or repeated washings with buffer, or both procedures, the leukocytes lose most of their reactivity to protein A, whereas the reactivity to anti‐IgE is unaltered. Cells deprived of their protein A reactivity can be induced to release histamine by IgG complexed with protein A. Maximal release (23%–81%) from 0.6–1.0 × 10 7 leukocytes per ml is obtained if the mixture contains 1–2 μg protein A and 8–16 μg IgG per ml. The ratio between protein A and IgG in the most active mixtures is 1:8 or 1:16 on a weight basis, which corresponds to 2–4 IgG molecules per protein A molecule. Heat treatment does not destroy the capacity of IgG to mediate histamine release. Mixtures of protein A and the Fc part of IgG can also initiate the release. Furthermore, it is shown that the protein A‐IgG mixture and anti‐IgE induce cross‐desensitization to each other. This indicates that, like cell‐bound IgG, IgG in complex with protein A triggers partially the same reaction sequence as IgE.