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Correction: Mouse Spleen Cells Cultured in Homologous Serum Can Indeed Make Primary Antibodies to Sheep Erythrocytes
Author(s) -
BRITTON S.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02676.x
Subject(s) - antibody , polyclonal antibodies , antigen , in vitro , homologous chromosome , spleen , biology , immunology , suppressor , microbiology and biotechnology , biochemistry , gene
Contrary to what I have reported in a previous article. I now present data that prove that spleen cells from various strains of mice can make primary antibodies In sheep erythrocytes in syngeneic adult serum The reasons for this dichotomy of data are not clear. The present results indicate that it is possible to obtain an antibody response in the absence of demonstrable mitogens or polyclonal activators, suggesting activating signals only between antigen‐specific cells Primary IgC formation in vitro was demonstrable in cultures with normal mouse serum but not in fetal‐calf‐serum‐substituted cultures. Since antigen has never induced antibody formation in vitro under serum‐free conditions, it is now deduced that normal adult serum, in addition to containing suppressor molecules that restrain general lymphocyte proliferation, also contains material absolutely essential for antigen‐induced specific cell proliferation to occur. These latter substances work even at very high dilutions.