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Detection of IgG Aggregates or Immune Complexes Using Solid‐Phase C1q and Protein A‐Rich Staphylococcus aureus as an Indicator System
Author(s) -
FARRELL C,
SØGAARD H,
SVEHAG S.E.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02675.x
Subject(s) - radioimmunoassay , staphylococcus aureus , chemistry , antigen , protein a , antibody , chromatography , immunoglobulin g , bovine serum albumin , antigen antibody complex , protein g , immune system , albumin , biochemistry , biology , immunology , bacteria , genetics
A radioimmunoassay for detection of Clq‐binding IgG aggregates and antigen‐IgG antibody complexes is described The assay makes use of solid‐phase Clq and 32 p‐labelled protein A‐rich Stphylococcusaureus as an indicator system. Both 19S and heavier IgG aggregates that fixed Clq were detected The sensitivity of the assay permitted detection of heavy (19–25S) IgG aggregates at a concentration of 8 μg/ml or less. The results indicated that detection of IgG in this assay is dependent on the degree of IgG polymerization and the molar ratio between the solid‐phase Clq and the IgG polymers. Albumin‐anti‐albumin complexes, preformed at equilibrium with antibody to antigen molar ratios of 2:1 to 3:1 and at antigen concentrations of 25 to 40 μ g/ml. were also detectable using the described radioimmunoassay