Premium
Quantitative Separation of Antigen‐Specific Murine Antibodies by Anti‐Allotype Chromatography
Author(s) -
FREED J. H.,
HERZENBERG L. A.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02653.x
Subject(s) - allotype , antiserum , antibody , antigen , affinity chromatography , microbiology and biotechnology , sepharose , chemistry , biology , chromatography , immunology , biochemistry , enzyme
The use of Sepharose‐conjugated murine anti‐Ig a or anti‐Ig b allo‐antisera allowed the quantitative separation of immunoglobulins of the two allotypes. After fractionation of mixtures of anti‐(T,G)‐A‐ ‐L antisera obtained from congenic strains differing in immunoglobulin allotype, it was possible to measure the antigen‐binding capacity of specific anti‐(T,G)‐A‐ ‐L antibodies in each allotype fraction. Analysis of artificial mixtures of immune sera obtained from homozygous Ig a and Ig b animals showed that this method is quantitative and internally consistent. This method of affinity chromatography was used in the analysis of specific anti‐(T,G)‐A–L antisera from tetraparental mice.