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Surface Markers on Human B and T Lymphocytes
Author(s) -
JONDAL M.,
KLEIN E.,
YEFENOF E.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02625.x
Subject(s) - neuraminidase , incubation , microbiology and biotechnology , trypsinization , surface immunoglobulin , rosette formation , lymphoblast , biology , cytotoxicity , cell , rosette (schizont appearance) , chemistry , antibody , immunology , cell culture , biochemistry , b cell , trypsin , in vitro , virus , enzyme , genetics
Almost 100% of peripheral T lymphocytes are shown to have the capacity to form rosettes with human lymphoblastoid B‐cell lines, predominantly at 4°C and with lines having surface‐bound IgG. Blast‐transformed T cells retained this capacity and formed rosettes even at 37°C Unstimulated T cells bound less readily to B‐cell blasts, stimulated by pokeweed mitogen for 72 hr. Even though rosettes, formed at 4°C, were stable for several hours at 72°C, no T‐cell‐mediated cytotoxicity could be detected during overnight incubation. Extreme pH values and trypsinization decreased rosette formation, whereas neuraminidase treatment enhanced the reaction. Rosette formation was independent of bivalent cations and unimpaired in the presence of inhibitors (NaF. NaN 3 ), undiluted human or fetal call sera, protein A, sonicated sheep erythrocyte membranes, and normal or heat‐aggregated human IgG. Anti‐Ig, anti β 2 ‐microglobulin, or anti‐T cell sera did not influence rosette formation.

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