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Effects of Treating Immune Lymphocytes with a Proteolytic Enzyme (Papain): Reduction in Immune Potential and Recovery after Incubation
Author(s) -
CROSS A. M.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02622.x
Subject(s) - papain , incubation , immune system , hapten , proteolytic enzymes , spleen , antibody , antigen , enzyme , incubation period , immunology , chemistry , biology , microbiology and biotechnology , biochemistry
Immune spleen cells were treated with papain, washed, and boosted with homologous antigen (4‐hydroxy‐3‐iodo‐5‐nitrophenylacetic acid coupled to chicken globulin) for 1 hr at 4°C. When transferred to irradiated mice, they produced up to 20‐fold less anti‐hapten antibody than non‐papain‐treated cells. The lymphocytes could recover their ability to respond if incubated at 37°C after papain treatment but before antigen boosting. Incubation for 30 min at 37°C gave complete or almost complete recovery. Although papain usually reduced the response, in 31% of experiments the reduction was not significant. Possible reasons for this variability are discussed.