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Genetic Control of the In Vitro Responses of Rat Blood Lymphocytes
Author(s) -
NIELSEN H. E.,
KOCH C.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02597.x
Subject(s) - in vitro , in vivo , biology , congenic , stimulation , lymphocyte , immunology , microbiology and biotechnology , chemistry , genetics , gene , endocrinology
In vitro DNA synthetic responses of blood lymphocytes from the inbred rat strains BN, Lewis, and AS were compared with each other after stimulation with allogeneic lymphocytes, human lymphocytes, phytohemagglutinin, and anti‐Ig antibodies. BN lymphocytes responded less well than AS and Lewis lymphocytes regardless of the stimulus used. The responses of the congenic strains Lewis and Lewis.BN were identical, suggesting that H‐1 (Ag‐B)‐linked loci do not determine in vitro responsiveness in these strains. In vivo, AS and BN rats were equally able to reject third‐party skin grafts and to induce graft‐versus‐host reactions in F 1 hybrids. We conclude that the interstrain differences found in vitro reflect genetically determined differences in in vitro kinetics, possibly related to the fraction of short‐lived lymphocytes present rather than to demonstrable differences in the frequency of reactive lymphocytes.