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‘Nonspecific’Blocking of Human Ovarian Carcinoma‐Associated Cellular Cytotoxicity In Vitro
Author(s) -
SAKSELA E.,
PENTTINEN K.,
PYRHÖNEN S.
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01313.x
Subject(s) - cytotoxic t cell , cytotoxicity , toxoid , effector , in vitro , ovarian carcinoma , chemistry , microbiology and biotechnology , receptor , cancer research , biology , immunology , antibody , ovarian cancer , biochemistry , immunization , cancer , genetics
The blocking ability of heat‐aggregated human polyclonal IgG and complexes formed by preservative‐free tetanus toxoid (TT) and human anti‐tetanus serum was investigated on the cell‐mediated tumor‐specific cytotoxicity reaction of ovarian carcinoma patients against cultured ovarian carcinoma cells. Aggregated IgG effectively blocked the cytotoxic reaction when the purified effector cells were prein‐cubated with various concentrations of this material, whereas no blocking activity was detected when target cells were preincubated and washed before addition of cytotoxic lymphocytes. Optimal concentration was about 1.6 μg/ml, and higher or lower concentrations were less effective. Anti‐TT/TT immunocomplexes behaved similarly and could effectively block at the effector cell but not at the target cell level. The results suggest that the effector cells in human ovarian carcinoma‐associated cellular cytotoxicity are Fc‐receptor‐carrying cells and that their cytotoxic activity can be ‘nonspecifically’ blocked by saturating these sites with aggregated IgG or unrelated immunocomplexes.