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Strong Stimulation of CBA Lymphocytes in the Mixed Lymphocyte Interaction with Cells from the H‐2‐Identical Strain C3H
Author(s) -
LILLIEHÖÖK B.,
BLOMGREN H
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01296.x
Subject(s) - spleen , stimulation , antigen , lymphocyte , immunology , in vitro , microbiology and biotechnology , biology , transplantation , t lymphocyte , strain (injury) , bone marrow , c57bl/6 , andrology , endocrinology , medicine , genetics , anatomy
Lymphocyt e! from the H‐2‐identical mouse strains CBA and C3H were tested for mixed lymphocyte culture (MLC) responsiveness in vitro. It was observed that CBA lymphocytes were stimulated by C3H cells to the same extent as or stronger than cells from H‐2‐disparate donors. C3H lymphocytes, however, were stimulated poorly by CBA cells. The stimulatory effect by C3Hcells of CBA lymphocytes was observed to be positively correlated with the age of the C3H donor. A similar but less pronounced difference was found by varying the age of donors for H‐2 disparate cells. Spleen cells from normal and thymectomized, irradiated, and bone marrow‐reconstituted C3H × CBA mice stimulated CBA lymphocytes to approximately the same extent, indicating that the stimulatory antigen is present on both T and B cells or possibly other cell types However, they arc poorly expressed or absent on thymic cells. It is concluded that certain non‐H‐2‐determined transplantation antigens may be strongly stimulatory in MLC tests.