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Primary Antibody Response by Chicken Spleen Cells In Vitro
Author(s) -
TUFVESON G.,
LUNDBERG I.,
ALM G V
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01292.x
Subject(s) - spleen , antibody response , antibody , in vitro , primary and secondary antibodies , immunology , stimulation , biology , immune system , endocrinology , biochemistry
An in vitro method that permits the primary antibody response of chicken spleen cells to sheep erythrocytes to occur is described, and the characteristic of the antibody response are given. Significant but variable plaque‐forming cell (PFC) responses were obtained—that is, some animals showed no or a very weak response In these the addition of small numbers of allogeneic spleen cells much improved the PFC responses. The characteristics of this allogeneic stimulation are described. The presented culture method should facilitate studies of cell interactions in the primary antibody response of the chicken and in the development of the antibody‐forming cells.