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Human Secretory Component
Author(s) -
BRANDTZAEG P
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01291.x
Subject(s) - chromatography , size exclusion chromatography , fractionation , antiserum , yield (engineering) , filtration (mathematics) , ultrafiltration (renal) , chemistry , ammonium , precipitation , component (thermodynamics) , ammonium sulfate precipitation , ammonium sulfate , biochemistry , antibody , biology , immunology , enzyme , materials science , organic chemistry , statistics , mathematics , physics , meteorology , metallurgy , thermodynamics
A new purification method is based on a combination of conventional fractionation procedures (ammonium sulphate precipitation, gel filtration, and anionic‐exchange chromatography) and immunological removal of contaminating proteins. The immunosorbent was prepared by polymerizing antisera to human serum and colostrum and was used in a batch procedure. By this method it was possible to obtain secretary component of about 98% purity in 10% yield (approximately 100 mg/l colostrums).