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Isolation of a Fragment, Fh, Corresponding to the Hinge Region of Human IgG3
Author(s) -
MICHAELSEN T E,
NATVIG J. B.,
SLETTEN K
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01282.x
Subject(s) - fragment (logic) , chymotrypsin , cystine , yield (engineering) , chemistry , hinge , proline , trypsin , stereochemistry , biochemistry , amino acid , microbiology and biotechnology , enzyme , biology , cysteine , materials science , physics , classical mechanics , metallurgy , computer science , programming language
An Fh fragment corresponding to the hinge region of IgG 3 has been isolated by J single α‐chymotrypsin treatment of whole IgG 3 Various combinations of two enzymes have also been used, but with somewhat lower yield and less purity The isolated Fh fragment has features similar to those of the bound hinge region in the intact IgG 3 and its F(ab ' ) 2 and Fch fragments. The Fh fragment has a char acteristically high content of cystine and proline and seems to lack aromatic amino acids