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Idiotypic Cross‐Reactions Among Anti‐Streptococcal Antibodies in an Inbred Rabbit Population
Author(s) -
KLAPPER D. C.,
KINDT T. J.
Publication year - 1974
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1974.tb01281.x
Subject(s) - antibody , antiserum , population , sepharose , immunoglobulin idiotypes , chemistry , cross reactions , biology , idiotype , microbiology and biotechnology , immunology , biochemistry , enzyme , monoclonal antibody , medicine , environmental health
Idiotypic cross‐reactions were detected among Group C streptococcal antibodies from 58% of related rabbits but were seldom seen among the antibodies of unrelated rabbits The studies reported here indicate that idiotypic cross‐reactions were due to a minor antibody component in each reactive streptococcal antiserum. Immunoadsorbent methods were modified to isolate antibody components from antisera on the basis of relative binding affinity. We used an immunoadsorbent prepared by coupling Ba(OH) 2 ‐treated Group C streptococcal carbohydrate to Sepharose with CNBr at pH 7. Three or four antibody components were recovered from each of four Group C antisera. Components containing idiotypically cross‐reacting antibodies represented 2% to 8% of the total serum antibody. Antibodies in the other components did not give idiotypic cross‐reactions. Light chains from the cross‐reacting antibody all displayed the same V x III amino terminal sequence. Ile‐Val‐Met. Such findings suggest linkage of this rare L chain variable region subgroup to this particular idiotypic specificity.